Ribosomal Precursor RNA in Saccharomyces carlsbergensis
نویسندگان
چکیده
منابع مشابه
Extrachromosomal circular ribosomal DNA in the yeast Saccharomyces carlsbergensis
Purified ribosomal DNA from Saccharomyces carlsbergensis contains a small proportion of circular DNA molecules with a contour length of 3 micron or integral multiples thereof. Hybridization of yeast ribosomal DNA with 26 S rRNA, using the R-loop technique, reveals that these circular molecules contain sequences complementary to yeast ribosomal RNA. We suggest that these extrachromosomal rRNA ge...
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The pseudouridylation of ribosomal RNA of Saccharomyces carlsbergensis was investigated with respect to its timing during the maturation of rRNA and its sequence specificity. Analysis of 37-S RNA, the common precursor to 17-S, 5.8-S and 26-S rRNA and most probably the primary ribosomal transcript, shows that this RNA molecule contains already most if not all of the 36-37 pseudouridine residues ...
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We have characterized a putative precursor RNA (15.5S) for the 15S ribosomal RNA in mitochondria of Saccharomyces cerevisiae. Hybrids were formed with mitochondrial RNA and mtDNA fragments terminally labelled at restriction sites located within the gene coding for 15S ribosomal RNA and treated with S1 nuclease (Berk, A.J. and Sharp, J.A. (1977) 12, 721-732). Sites of resistant hybrids were meas...
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Cleavage of the Saccharomyces cerevisiae primary ribosomal RNA (rRNA) transcript in the 3' external transcribed spacer (ETS) by Rnt1p generates the 35S pre-rRNA, the earliest detectable species in the pre-rRNA processing pathway. In this study we show that Rnt1p is concentrated in a subnucleolar dot-shaped territory distinct from the nucleolar body. The 35S pre-rRNA is localized at the peripher...
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A particulate preparation obtained by lysing yeast protoplasts was found to catalyze the incorporation of radioactivity from guanosine diphosphate 14C-mannose into a mannose polysaccharide. The k, for guanosine diphosphate mannose is 0.5 mu, and the optimum pH is between 5.5 and 7.2. There is an almost absolute requirement of Mnff for activity, which cannot be substituted by Mg++. The reaction ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1967
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1967.tb19524.x